ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (11): 1746-1751.doi: 10.11843/j.issn.0366-6964.2014.11.002

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NOBOX Gene Expression in Maiwa Yak Oocytes,Early Embryogenesis and Fetal Ovaries

FU Mei1#,CHEN Ya-bing1#,LI Jian2*,XIONG Xian-rong1,LAN Dao-liang2   

  1. (1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China;2.College of Tibetan Plateau Research,Southwest University for Nationalities,Chengdu 610041,China)
  • Received:2014-05-16 Online:2014-11-23 Published:2014-11-23

Abstract:

The objective of this study was to investigate the correlation between yak NOBOX gene expression and oocytes,embryogenesis and fetal ovaries.RT-PCR was used to detect the expression pattern of NOBOX gene in yak fetal ovaries,granulose cells and various tissues;Real-time PCR was chosen to analyze the dynamic changes of NOBOX gene expression during oocyte maturation and early embryonic development (GV,MII,2-cell,4-cell,8-cell,16-cell,MO and BL).The results showed that NOBOX gene was expressed in various tissues of yak,and highest in kidney.At GV-stage ovarian follicle,the expression of NOBOX gene was detected only in oocytes not in granule cells.Moreover,NOBOX gene was expressed regularly during early embryonic development,which has a highest level at GV-stage (P<0.05),decreasing with the embryonic development,and attained a steady level at 16-cell stage.However,it’s noteworthy that the expression of NOBOX gene in ovary increased with yak fetal growth and development.The different expression pattern of NOBOX gene in different stages of early embryonic development may be associated with embryonic development and various physiological activities.

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